Es tee oh see kay. You're trying to do more work than necessary. Start by going back to stock. If it goes away, try your custom tune again. If it follows, it's the tune. It makes the most sense. Again, its free, and quick and will narrow down the possibilities.

 

I got a update on my truck today. I got the spark plugs and boots put in. and now the injector on cylinder #6 is having issues in Drive. the pulse is brighter on injector 6 and causing cylinder 6 miss fire